![]() The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. Lane 4 : PC12 (Rat adrenal gland pheochromocytoma cell line) Whole Cell LysateĪll lanes : Goat Anti-Mouse IgG H&L (Alexa Fluor® 790) ( ab175783) at 1/10000 dilution Lane 3 : NIH 3T3 (Mouse embryo fibroblast cell line) Whole Cell Lysate Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate Lane 1 : A431 (Human epidermoid carcinoma cell line) Whole Cell Lysate Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&AsĪll lanes : Anti-beta Actin antibody - Loading Control (ab8226) at 1/1000 dilution If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Please check that this product meets your needs before purchasing. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. The Life Science industry has been in the grips of a reproducibility crisis for a number of years. If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact you can find further information here. This antibody clone is manufactured by Abcam. Contact our Scientific Support team for more information or advice. If milk block is required, we recommend using ab8224 mouse monoclonal to beta actin. Please see the comparison data in the images section. We recommend blocking with 2-5% BSA as we have found that use of 5% milk significantly reduces the band intensity for beta actin.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |